Product Type: Antibody
Descritpion: This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms.
Research Areas: Cancer, Signal transduction
Recommended Dilutions: WB 1:500-2000, IHC 1:25-100, ELISA 1:2000-5000
Size: 200 uL
Gene Accession: NP_055018
Immunogen: Synthetic peptide of human AP2A1
Formulation: PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Calculated MW: 108 kDa
Tissue Specificity/Positive Control: Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
Cellular Localization: Cell membrane. Membrane>coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Storage: Store at -20C. Avoid freeze / thaw cycles.