Phospho-PTEN (Ser370) Polyclonal AntibodyE-AB-20974
Product Category: Phospho/Acetyl/Methyl
Description: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Size: 200 uL
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000
Gene ID: 5728
Immunogen: Synthesized peptide derived from human PTEN around the phosphorylation site of Ser370
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Secreted. May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif and Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.
Product Link: https://www.elabscience.com/p-phospho_pten_(ser370)_polyclonal_antibody-25563.html
Manaul Link: https://www.elabscience.com/viewpdf-25563-elabscience-E-AB-20974.PDF