Phospho-SHC1 (Tyr349) Polyclonal AntibodyE-AB-21123
Product Category: Phospho/Acetyl/Methyl
Description: Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.
Research Areas: Cancer, Neuroscience, Signal transduction
Size: 200 uL
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Gene ID: 6464
Immunogen: Synthesized peptide derived from human Shc around the phosphorylation site of Tyr349
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Observed MW: 66, 52, 46kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm; Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization and Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
Product Link: https://www.elabscience.com/p-phospho_shc1_(tyr349)_polyclonal_antibody-25712.html
Manaul Link: https://www.elabscience.com/viewpdf-25712-elabscience-E-AB-21123.PDF