Phospho-AKT1/3 (Tyr437/434) Polyclonal AntibodyE-AB-21222
Product Category: Phospho/Acetyl/Methyl
Description: AKT1 and AKT3 are members of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet derived growth factor (PDGF), insulin, and insulin like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. The protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3 kinase. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Size: 200 uL
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Gene ID: 207/10000
Immunogen: Synthesized peptide derived from human Akt1/3 around the phosphorylation site of Tyr437/434
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Observed MW: 56kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: AKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
Product Link: https://www.elabscience.com/p-phospho_akt1_3_(tyr437_434)_polyclonal_antibody-25811.html
Manaul Link: https://www.elabscience.com/viewpdf-25811-elabscience-E-AB-21222.PDF