Phospho-STAT1 (Ser727) Polyclonal AntibodyE-AB-21252
Product Category: Phospho/Acetyl/Methyl
Description: Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal transduction
Size: 200 uL
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Gene ID: 6772
Immunogen: Synthesized peptide derived from human Stat1 around the phosphorylation site of Ser727
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Observed MW: 87kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
Product Link: https://www.elabscience.com/p-phospho_stat1_(ser727)_polyclonal_antibody-25841.html
Manaul Link: https://www.elabscience.com/viewpdf-25841-elabscience-E-AB-21252.PDF