Phospho-NF2 (Ser10) Polyclonal Antibody

Supplier: Elabscience
Product Category: Phospho/Acetyl/Methyl
Description: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Signal transduction
Size: 200 uL
Concentration: 1mg/mL
Applications: WB,IHC-p,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000
Clonality: Polyclonal
Clone No:
SwissProt: P35240
Gene ID: 4771
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from human NF2 around the phosphorylation site of Ser10
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Observed MW: 65kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm>perinuclear region. Cytoplasmic granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia; Cytoplasm>perinuclear region. Cytoplasmic granule. Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia; Nucleus. Cell projection>filopodium membrane. Cell projection>ruffle membrane. Cytoplasm>perinuclear region. Cytoplasmic granule. Cytoplasm>cytoskeleton. In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia and Cell projection>filopodium membrane. Cell projection>ruffle membrane. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.

Product Link: https://www.elabscience.com/p-phospho_nf2_(ser10)_polyclonal_antibody-41106.html
Manaul Link: https://www.elabscience.com/viewpdf-41106-elabscience-E-AB-21459.PDF