NLRP3 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. 
Research Areas: Cancer, Cell Biology, Cardiovascular, Immunology, Signal transduction
Applications: WB,IHC-p,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000-1:20000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: Q96P20
Gene Accession:
Reactivity: Human
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the Internal region of human Cryopyrin.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 114kDa
Tissue Specificity/Positive Control: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Cellular Localization: Cytoplasm.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-nlrp3_polyclonal_antibody-26454.html
Manual Link: https://www.elabscience.com/viewpdf-26454-elabscience-E-AB-30129.PDF