Ub Polyclonal AntibodyE-AB-30177
Product Type: Antibody
Descritpion: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:2000, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from human Ub around the non-acetylation site of K63.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Tissue Specificity/Positive Control:
Cellular Localization: Cytosolic large ribosomal subunit,Endoplasmic reticulum,endoplasmic reticulum,endoplasmic reticulum membrane,endoplasmic reticulum quality control compartment,Endosome membrane,Extracellular region or secreted,extracellular exosome,extracellular space,Lysosome,lysosomal membrane,Mitochondrion,mitochondrial outer membrane,Nucleus,nucleoplasm,nucleus,Plasma Membrane,Other locations:endocytic vesicle membrane,host cell,vesicle
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-ub_polyclonal_antibody-26502.html
Manual Link: https://www.elabscience.com/viewpdf-26502-elabscience-E-AB-30177.PDF