HIF1 alpha Polyclonal AntibodyE-AB-30330
Product Type: Antibody
Descritpion: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Recommended Dilutions: WB 1:1000-2000
Size: 200 uL
Immunogen: Recombinant Protein
Formulation: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification: Affinity purification
Tissue Specificity/Positive Control: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
Cellular Localization: Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-hif1_alpha_polyclonal_antibody-26655.html
Manual Link: https://www.elabscience.com/viewpdf-26655-elabscience-E-AB-30330.PDF