PAK1/2/3 Polyclonal AntibodyE-AB-32500
Product Type: Antibody
Descritpion: In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Research Areas: Cancer, Cell Biology, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from human PAK?/?/? around the non-phosphorylation site of T423/402/421.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Tissue Specificity/Positive Control: PAK1 is Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).PAK2 isUbiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.PAK3 is Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
Cellular Localization: Cytosol,Nucleus,nuclear membrane,Plasma Membrane,ruffle membrane,Other locations:axon,cell-cell junction,cytoplasm,dendrite,focal adhesion,intercalated disc,invadopodium,lamellipodium,protein complex,ruffle,Z disc
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-pak1_2_3_polyclonal_antibody-28825.html
Manual Link: https://www.elabscience.com/viewpdf-28825-elabscience-E-AB-32500.PDF