Product Type: Antibody
Descritpion: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Research Areas: Cancer, Immunology, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Tyr458/199.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 54+83kDa
Tissue Specificity/Positive Control: PI 3 kinase p85 alpha is Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).1 PublicationPI 3 kinase p85 gamma isHighest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Cellular Localization: Cytoplasmic
Storage: Store at -20C. Avoid freeze / thaw cycles.