PRKAR2B Polyclonal AntibodyE-AB-32596
Product Type: Antibody
Descritpion: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.PRKAR2B (Protein Kinase CAMP-Dependent Type II Regulatory Subunit Beta) is a Protein Coding gene. Among its related pathways are CCR5 Pathway in Macrophages and Aquaporin-mediated transport. An important paralog of this gene is PRKAR2A.
Research Areas: Cancer, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Size: 200 uL
Immunogen: Synthesized peptide derived from human PKA II? reg around the non-phosphorylation site of Ser113.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 46kDa
Tissue Specificity/Positive Control: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Cellular Localization: Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-prkar2b_polyclonal_antibody-28921.html
Manual Link: https://www.elabscience.com/viewpdf-28921-elabscience-E-AB-32596.PDF