RACGAP1 Polyclonal AntibodyE-AB-32715
Product Type: Antibody
Descritpion: This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.
Research Areas: Cancer, Cell Biology, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:40000
Size: 200 uL
Immunogen: Synthesized peptide derived from human Rac GAP1 around the non-phosphorylation site of Ser387.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 71kDa
Tissue Specificity/Positive Control: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
Cellular Localization: Nucleus. Cytoplasm. Cytoplasm>cytoskeleton>spindle. Cytoplasmic vesicle>secretory vesicle>acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-racgap1_polyclonal_antibody-29040.html
Manual Link: https://www.elabscience.com/viewpdf-29040-elabscience-E-AB-32715.PDF