RAD17 Polyclonal AntibodyE-AB-32719
Product Type: Antibody
Descritpion: Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Research Areas: Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Size: 200 uL
Immunogen: Synthesized peptide derived from human Rad17 around the non-phosphorylation site of S645.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Calculated MW: 77kDa
Tissue Specificity/Positive Control: Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Cellular Localization: Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-rad17_polyclonal_antibody-29044.html
Manual Link: https://www.elabscience.com/viewpdf-29044-elabscience-E-AB-32719.PDF