RAD50 Polyclonal AntibodyE-AB-32722
Product Type: Antibody
Descritpion: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from the Internal region of human Rad50
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 154kDa
Tissue Specificity/Positive Control: Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Cellular Localization: Nucleus. Chromosome>telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-rad50_polyclonal_antibody-29047.html
Manual Link: https://www.elabscience.com/viewpdf-29047-elabscience-E-AB-32722.PDF