ROCK1 Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Research Areas: Cancer, Cell Biology, Signal transduction
Applications: WB,IHC-p,IF,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ICC 1:200-1:1000, IF 1:50-1:200, ELISA 1:10000-1:20000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: Q13464
Gene Accession:
Reactivity: Human,Mouse,Rat,Monkey
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the Internal region of human Rock-1.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 158kDa
Tissue Specificity/Positive Control: Detected in blood platelets.
Cellular Localization: Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-rock1_polyclonal_antibody-29149.html
Manual Link: https://www.elabscience.com/viewpdf-29149-elabscience-E-AB-32824.PDF