SGO1 Polyclonal AntibodyE-AB-32885
Product Type: Antibody
Descritpion: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. SGO1 (Shugoshin 1) is a Protein Coding gene. Diseases associated with SGO1 include Chronic Atrial And Intestinal Dysrhythmia and Sick Sinus Syndrome. Among its related pathways are PLK1 signaling events and Aurora B signaling.
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from the Internal region of human Sgo1
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Calculated MW: 64kDa
Tissue Specificity/Positive Control: Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
Cellular Localization: Nucleus. Chromosome>centromere. Chromosome>centromere>kinetochore. Cytoplasm>cytoskeleton>spindle pole. Cytoplasm>cytoskeleton>centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-sgo1_polyclonal_antibody-29210.html
Manual Link: https://www.elabscience.com/viewpdf-29210-elabscience-E-AB-32885.PDF