SHANK2 Polyclonal AntibodyE-AB-32887
Product Type: Antibody
Descritpion: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants.SHANK2 (SH3 And Multiple Ankyrin Repeat Domains 2) is a Protein Coding gene. Diseases associated with SHANK2 include Autism Susceptibility 17 and Autism Spectrum Disorder. Among its related pathways are Protein-protein interactions at synapses and Neuroscience. GO annotations related to this gene include SH3 domain binding and GKAP/Homer scaffold activity. An important paralog of this gene is SHANK3.
Research Areas: Neuroscience
Recommended Dilutions: WB 1:500-1:2000, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from the Internal region of human Shank 2
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Calculated MW: 135kDa
Tissue Specificity/Positive Control: Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
Cellular Localization: Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-shank2_polyclonal_antibody-29212.html
Manual Link: https://www.elabscience.com/viewpdf-29212-elabscience-E-AB-32887.PDF