MYO6 Polyclonal AntibodyE-AB-33342
Product Type: Antibody
Descritpion: This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. MYO6 (Myosin VI) is a Protein Coding gene. Diseases associated with MYO6 include Deafness, Autosomal Dominant 22 and Deafness, Autosomal Recessive 37. Among its related pathways are PAK Pathway and Vesicle-mediated transport. GO annotations related to this gene include actin binding and actin filament binding. An important paralog of this gene is MYO7A.
Research Areas: Cancer, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-300, ELISA 1:5000
Size: 200 uL
Immunogen: Synthesized peptide derived from the N-terminal region of human Myosin VI
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 150kDa
Tissue Specificity/Positive Control: Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.
Cellular Localization: Cytoplasmic vesicle>clathrin-coated vesicle membrane; Cytoplasmic vesicle>clathrin-coated vesicle membrane. Cell projection>ruffle membrane and Golgi apparatus>trans-Golgi network membrane. Golgi apparatus. Nucleus. Cytoplasm>perinuclear region. Membrane>clathrin-coated pit. Cell projection>ruffle membrane. Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage. Recruited into membrane ruffles from cell surface by EGF-stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-myo6_polyclonal_antibody-29667.html
Manual Link: https://www.elabscience.com/viewpdf-29667-elabscience-E-AB-33342.PDF