HGS Polyclonal AntibodyE-AB-33422
Product Type: Antibody
Descritpion: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Research Areas: Cancer, Cell Biology, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, ELISA 1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from the Internal region of human Hrs.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Calculated MW: 86kDa
Tissue Specificity/Positive Control: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
Cellular Localization: Cytoplasm. Early endosome membrane. Endosome>multivesicular body membrane.
Storage: Store at -20C. Avoid freeze / thaw cycles.
Product Link: https://www.elabscience.com/p-hgs_polyclonal_antibody-29747.html
Manual Link: https://www.elabscience.com/viewpdf-29747-elabscience-E-AB-33422.PDF