Product Type: Antibody
Descritpion: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NF?B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ?B sequence in the immunoglobulin ? light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF?B is activated and NF?B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
Research Areas: Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal transduction
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000-1:20000
Size: 200 uL
Immunogen: Synthesized peptide derived from human NF?B-p65 around the non-acetylation site of K218.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Calculated MW: 60kDa
Tissue Specificity/Positive Control:
Cellular Localization: Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
Storage: Store at -20C. Avoid freeze / thaw cycles.