Ub Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Applications: WB,IHC-p,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300 ELISA 1:10000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: P62987,P62979,P0CG47,P0CG48
Gene Accession:
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the N-terminal region of human Ub.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW:
Tissue Specificity/Positive Control:
Cellular Localization: Cytosolic large ribosomal subunit,Endoplasmic reticulum,endoplasmic reticulum,endoplasmic reticulum membrane,endoplasmic reticulum quality control compartment,Endosome membrane,Extracellular region or secreted,extracellular exosome,extracellular space,Lysosome,lysosomal membrane,Mitochondrion,mitochondrial outer membrane,Nucleus,nucleoplasm,nucleus,Plasma Membrane,Other locations:endocytic vesicle membrane,host cell,vesicle
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-ub_polyclonal_antibody-29943.html
Manual Link: https://www.elabscience.com/viewpdf-29943-elabscience-E-AB-33618.PDF