Mouse HIF-1a (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
Mouse HIF-1a (Hypoxia Inducible Factor 1 Alpha) CLIA Kit

Mouse HIF-1a (Hypoxia Inducible Factor 1 Alpha) CLIA Kit


Regular price $650.00 $553.00 You Pay

Supplier: Elabscience
Product Type: CLIA
Size: 96T
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1? . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1?, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HIF-1?. The concentration of Mouse HIF-1? in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: HIF-1?
Target Synonym: HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor
UNIProt ID: Q61221
Target Species: Mouse
Type: Sandwich
Detection Range: 31.25--2000 pg/mL
Detection Sensitivity: 18.75 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
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