Product Type: CLIA
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1? . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1?, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-1?. The concentration of Rat IL-1? in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: IL-1?
Target Synonym: IL1B; IL1-BETA; IL1F2; catabolin
UNIProt ID: Q63264
Target Species: Rat
Detection Range: 12.5--800 pg/mL
Detection Sensitivity: 7.5 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-rat_il_1?_(interleukin_1_beta)_clia_kit-16950.html
Manual Link: http://www.elabscience.com/viewpdf-16950-Elabscience-E-CL-R0012.pdf