Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit

Supplier: Elabscience
Product Type: CLIA
Size: 96T
Description: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NOS3/eNOS. The concentration of Rat NOS3/eNOS in the samples can be calculated by comparing the RLU of the samples to the standard curve.
Target Name: NOS3/eNOS
Target Synonym: ECNOS; NOS III; E-NOS
UNIProt ID: Q62600
Target Species: Rat
Type: Sandwich
Detection Range: 7.813--500 pg/mL
Detection Sensitivity: 4.688 pg/mL
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-rat_nos3_enos_(nitric_oxide_synthase_3,_endothelial)_clia_kit-17141.html
Manual Link: http://www.elabscience.com/viewpdf-17141-Elabscience-E-CL-R0237.pdf