cAMP (Cyclic adenosine monophosphate) ELISA Kit
cAMP (Cyclic adenosine monophosphate) ELISA Kit

cAMP (Cyclic adenosine monophosphate) ELISA Kit


Regular price $495.00 $420.00 You Pay

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of  cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: cAMP
Target Synonmy: Cyclic adenosine monophosphate
Target Species: Universal
Detection Range: 1.563--100ng/mL
Sensitivity: 0.938ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

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