12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of  12-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: 12-HETE
Target Synonmy: 12-Hydroxyeicosatetraenoic Acid
UNIProt ID:
Target Species: Universal
Detection Range: 0.78--50ng/mL
Sensitivity: 0.63ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-12_hete(12_hydroxyeicosatetraenoic_acid)_elisa_kit-40927.html

Manaul Link: http://www.elabscience.com/viewpdf-40927-Elabscience-E-EL-0109.pdf