Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Chicken GLP-1. During the reaction, Chicken GLP-1 in the sample or standard competes with a fixed amount of Chicken GLP-1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Chicken GLP-1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Chicken GLP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: GLP-1
Target Synonmy: Glucagon Like Peptide 1
UNIProt ID:
Target Species: Chicken
Detection Range: 78.125--5000pg/mL
Sensitivity: 46.875pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant
Product Link: http://www.elabscience.com/p-chicken_glp_1(glucagon_like_peptide_1)_elisa_kit-17633.html
Manaul Link: http://www.elabscience.com/viewpdf-17633-Elabscience-E-EL-Ch0160.pdf