Product Type: ELISA Kit
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse PGF2?. During the reaction, Mouse PGF2? in the sample or standard competes with a fixed amount of Mouse PGF2? on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PGF2?. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse PGF2? in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: PGF2?
Target Synonmy: Prostaglandin F2 Alpha
Target Species: Mouse
Detection Range: 15.625--1000pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant