Rat CRH (Corticotropin Releasing Hormone) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: CRH
Target Synonmy: Corticotropin Releasing Hormone
UNIProt ID: P01143
Target Species: Rat
Detection Range: 0.313--20ng/mL
Sensitivity: 0.188ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-rat_crh(corticotropin_releasing_hormone)_elisa_kit-17786.html

Manaul Link: http://www.elabscience.com/viewpdf-17786-Elabscience-E-EL-R0270.pdf