Rat fT4 (Free Thyroxine) ELISA Kit
Rat fT4 (Free Thyroxine) ELISA Kit

Rat fT4 (Free Thyroxine) ELISA Kit

E-EL-R0390

Regular price $495.00 $426.00 You Pay

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat fT4. During the reaction, Rat fT4 in the sample or standard competes with a fixed amount of Rat fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat fT4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: fT4
Target Synonmy: Free Thyroxine
UNIProt ID:
Target Species: Rat
Detection Range: 31.25--2000pg/mL
Sensitivity: 18.75pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-rat_ft4(free_thyroxine)_elisa_kit-17794.html

Manaul Link: http://www.elabscience.com/viewpdf-17794-Elabscience-E-EL-R0390.pdf