Rat HRH4(Histamine Receptor H4)ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HRH4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HRH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HRH4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HRH4. The concentration of Rat HRH4 in samples can be calculated by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Sandwich
Target Name: HRH4
Target Synonmy: Histamine Receptor H4
UNIProt ID: Q91ZY1
Target Species: Rat
Detection Range: 0.313--20ng/mL
Sensitivity: 0.188ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-rat_hrh4(histamine receptor h4)_elisa kit-22503.html

Manaul Link: http://www.elabscience.com/viewpdf-22503-Elabscience-E-EL-R2556.pdf