Mouse Monoclonal Antibody to E2F1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human; Mouse
Clone: 8G9;
Isotype: Mouse IgG1
Immunogen: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli. ; ;
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 46.9kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=783
Reference: 1.Cancer Res. 2010 Dec 1;70(23):9711-20. ; 2.Mol Cancer Ther. 2010 May;9(5):1265-73. ;

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