Mouse Monoclonal Antibody to WT1

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 5G11A5;
Isotype: IgG1
Immunogen: Purified recombinant fragment of human WT1 (AA: 314-479) expressed in E. Coli.
Formulation: Purified antibody in PBS with 0.05% sodium azide.
MW: 49.2kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=3674
Reference: 1. Leuk Res. 2013 Oct;37(10):1341-9. ; 2. Pediatr Blood Cancer. 2013 Aug;60(8):1388-9.

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