Mouse Monoclonal Antibody to ATG5

Supplier: ProMab Technologies
Type of Product: Monoclonal Antibody
Description: ATG5 involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating; system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential; for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8; family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after; oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the; innate antiviral immune response by blocking the type I IFN production pathway through direct association with; RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation; apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T; lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II.; Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte; differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation; of IFT20 via the autophagic pathway;
Application: ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: ; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size: 100 ul, 1mg/ml
Species Reactivity: Human
Clone: 8E8G6;
Isotype: Mouse IgG2a
Immunogen: Synthesized peptide of human ATG5 (AA: MTDDKDVLRDVWFGRIc).
Formulation: Purified antibody in PBS with 0.05% sodium azide
MW: 32.4kDa
Storage: 4C; -20C for long term storage
Supplier link: http://www.promab.com/index.php?main_page=product_info&products_id=3736
Reference: Autophagy. 2013 Jan;9(1):20-32. ; Anticancer Res. 2012 Sep;32(9):4091-6.;

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