PKM Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Applications: WB,IHC-p,IF,ELISA
Recommended Dilutions: WB 1:500-2000, IHC 1:50-300, IF 1:50-1:200, ELISA 1:10000-20000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: P14618
Gene Accession:
Reactivity: Human,Mouse,Rat
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from the Internal region of human PKM2.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 58kDa
Tissue Specificity/Positive Control: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Cellular Localization: Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-pkm_polyclonal_antibody-28940.html
Manual Link: https://www.elabscience.com/viewpdf-28940-elabscience-E-AB-32615.PDF