PTEN Polyclonal Antibody

Supplier: Elabscience
Product Type: Antibody
Clonality: Polyclonal
Descritpion: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction
Applications: WB,IHC-p,ELISA
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000
Size: 200 uL
Concentration: 1mg/mL
Swissprot: P60484
Gene Accession:
Reactivity: Human,Mouse,Rat,Monkey
Host: Rabbit
Isotype: IgG
Immunogen: Synthesized peptide derived from human PTEN around the non-phosphorylation site of Ser370.
Formulation: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification: Affinity purification
Conjugation: Unconjugated
Calculated MW: 47kDa
Tissue Specificity/Positive Control: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Cellular Localization: Secreted. May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif and Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.
Storage: Store at -20C. Avoid freeze / thaw cycles.

Product Link: https://www.elabscience.com/p-pten_polyclonal_antibody-29004.html
Manual Link: https://www.elabscience.com/viewpdf-29004-elabscience-E-AB-32679.PDF