Human HNF1a (Hepatocyte Nuclear Factor 1 Alpha) ELISA Kit 

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HNF1a . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HNF1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HNF1a. The concentration of Human HNF1a in samples can be calculated by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Sandwich
Target Name: HNF1a
Target Synonmy: Hepatocyte Nuclear Factor 1 Alpha
UNIProt ID: P20823
Target Species: Human
Detection Range: 0.313--20ng/mL
Sensitivity: 0.188ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-human hnf1?(hepatocyte_nuclear_factor_1_alpha)_elisa_kit-20095.html

Manaul Link: http://www.elabscience.com/viewpdf-20095-Elabscience-E-EL-H5614.pdf