Mouse NN-T4 (Neonatal Thyroxine) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse NN-T4. During the reaction, Mouse NN-T4 in the sample or standard competes with a fixed amount of Mouse NN-T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse NN-T4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Mouse NN-T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: NN-T4
Target Synonmy: Neonatal Thyroxine
UNIProt ID:
Target Species: Mouse
Detection Range: 78.125--5000pg/mL
Sensitivity: 46.875pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-mouse_nn_t4(neonatal_thyroxine)_elisa_kit-17749.html

Manaul Link: http://www.elabscience.com/viewpdf-17749-Elabscience-E-EL-M0814.pdf