Mouse APLN (Apelin) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse APLN. During the reaction, Mouse APLN in the sample or standard competes with a fixed amount of Mouse APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse APLN. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: APLN
Target Synonmy: Apelin
UNIProt ID: Q9R0R4
Target Species: Mouse
Detection Range: 125--8000pg/mL
Sensitivity: 75pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-mouse_apln(apelin)_elisa_kit-17762.html

Manaul Link: http://www.elabscience.com/viewpdf-17762-Elabscience-E-EL-M2422.pdf