Rat DHEA-S7 (Dehydroepiandrosterone S7) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat DHEA-S7. During the reaction, Rat DHEA-S7 in the sample or standard competes with a fixed amount of Rat DHEA-S7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DHEA-S7. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat DHEA-S7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: DHEA-S7
Target Synonmy: Dehydroepiandrosterone S7
UNIProt ID:
Target Species: Rat
Detection Range: 3.906--250ng/mL
Sensitivity: 2.344ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-rat_dhea_s7(dehydroepiandrosterone_s7)_elisa_kit-17789.html

Manaul Link: http://www.elabscience.com/viewpdf-17789-Elabscience-E-EL-R0324.pdf