Recombinant Mouse FGFR3 / CD333 Protein (His & Fc tag)

Supplier: Elabscience Product Name: Recombinant Mouse FGFR3 / CD333 Protein (His & Fc tag) Catalog No. PKSM040942 Product Type: recombinant protein Size: 100 ug Activity: Measured by its ability to bind mouse aFGF in a functional ELISA. Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Tyr 367) of mouse FGFR3 (NP_032036.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Sequence: Met 1-Tyr 367 Fusion Tag: C-His & Fc Accession: NP_032036.2 Species: Mouse Expressed Host: HEK293 Cells Shipping Conditions: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Purity: > 92 % as determined by SDS-PAGE Endotoxin: < 1.0 EU per ?g of the protein as determined by the LAL method Stability: Samples are stable for up to twelve months from date of receipt at -70?.Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Molecular Weight: 66 kDa Applied MM: 100-110 kDa Formulation: Lyophilized from sterile PBS, pH 7.4 Reconstitution: Please refer to the printed manual for detailed information. Background: FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy