Dog Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Tumor Necrosis Factor Alpha Alternative Names: DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2 Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Cytokine;Tumor immunity;Infection immunity; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Organism Species: Canis familiaris; Canine (Dog) Detection Sensitivity: < 6.1pg/mL Detection Range: 15.6-1000pg/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEA133Ca.html