Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Apoptosis Signal Regulating Kinase 1
Alternative Names: MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Signal Regulating Kinase 1 (ASK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Signal Regulating Kinase 1 (ASK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Signal Regulating Kinase 1 (ASK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Enzyme & Kinase;Apoptosis;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: Tissue homogenates, cell lysates and other biological fluids
Organism Species: Homo sapiens (Human)
Detection Sensitivity: < 0.119ng/mL
Detection Range: 0.312-20ng/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SEB358Hu.html