Human Apolipoprotein M (APOM) ELISA Kit

Supplier: Cloud-Clone Corp. Product Type: ELISA Kit Size: 96T Item Name: Apolipoprotein M Alternative Names: Apo-M; G3a; NG20; Protein G3a Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein M (APOM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein M (APOM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein M (APOM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein M (APOM) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;Nutrition metabolism; Assay Type: Double-antibody Sandwich Applications: Enzyme-linked immunosorbent assay for Antigen Detection. Sample Types: Serum, plasma, tissue homogenates and other biological fluids Organism Species: Homo sapiens (Human) Detection Sensitivity: < 2.51ng/mL Detection Range: 1.56-100ng/mL Assay Length: 2 hours Product Link: http://www.cloud-clone.com/products/SEC299Hu.html