Supplier: Cloud-Clone Corp.
Product Type: ELISA Kit
Size: 96T
Item Name: Retinoschisin
Alternative Names: RS1; XLRS1; Retinoschisis X-Linked,Juvenile 1; X-linked juvenile retinoschisis protein
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoschisin (RS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoschisin (RS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoschisin (RS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoschisin (RS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Signal transduction;
Assay Type: Double-antibody Sandwich
Applications: Enzyme-linked immunosorbent assay for Antigen Detection.
Sample Types: Tissue homogenates, cell lysates and other biological fluids
Organism Species: Homo sapiens (Human)
Detection Sensitivity: < 0.057ng/mL
Detection Range: 0.156-10ng/mL
Assay Length: 2 hours
Product Link: http://www.cloud-clone.com/products/SEJ847Hu.html