8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: 8-OHdG
Target Synonmy: 8-Hydroxydeoxyguanosine
UNIProt ID:
Target Species: Universal
Detection Range: 1.563--100ng/mL
Sensitivity: 0.938ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-8_ohdg(8_hydroxydeoxyguanosine)_elisa_kit-40922.html

Manaul Link: http://www.elabscience.com/viewpdf-40922-Elabscience-E-EL-0028.pdf