3-NT (3-Nitrotyrosine) ELISA Kit
3-NT (3-Nitrotyrosine) ELISA Kit

3-NT (3-Nitrotyrosine) ELISA Kit

E-EL-0040

Regular price $495.00 $420.00 You Pay

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of  3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: 3-NT
Target Synonmy: 3-Nitrotyrosine
UNIProt ID:
Target Species: Universal
Detection Range: 1.563--100ng/mL
Sensitivity: 0.938ng/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-3_nt(3_nitrotyrosine)_elisa_kit-17597.html

Manaul Link: http://www.elabscience.com/viewpdf-17597-Elabscience-E-EL-0040.pdf