8-epi-PGF2? (8-Epi-Prostaglandin F2 Alpha) ELISA Kit

Supplier: Elabscience
Product Type: ELISA Kit
Size: 96T
Description: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2?. During the reaction, 8-epi-PGF2? in the sample or standard competes with a fixed amount of  8-epi-PGF2? on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  8-epi-PGF2?. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of   8-epi-PGF2? in the samples is then determined by comparing the OD of the samples to the standard curve.
Detection Type: TMB
Assay Type: Competitive
Target Name: 8-epi-PGF2?
Target Synonmy: 8-Epi-Prostaglandin F2 Alpha
UNIProt ID:
Target Species: Universal
Detection Range: 15.625--1000pg/mL
Sensitivity: 9.375pg/mL
Intended Samples: Serum, Plasma, Biological Fluids
Tested Samples: Serum, Plasma, Cell supernatant

Product Link: http://www.elabscience.com/p-8_epi_pgf2?(8_epi_prostaglandin_f2_alpha)_elisa_kit-17598.html

Manaul Link: http://www.elabscience.com/viewpdf-17598-Elabscience-E-EL-0041.pdf