Phospho-P53 (Ser366) Polyclonal AntibodyE-AB-21221
Product Category: Phospho/Acetyl/Methyl
Description: The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Size: 200 uL
Recommended Dilutions: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Gene ID: 7157
Immunogen: Synthesized peptide derived from human p53 around the phosphorylation site of S366.
Formulation: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purification: Affinity purification
Observed MW: 48kDa
Storage: Store at -20C. Avoid freeze / thaw cycles.
Cellular Localization: Cytoplasm; Cytoplasm. Nucleus. Nucleus>PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.
Product Link: https://www.elabscience.com/p-phospho_p53_(ser366)_polyclonal_antibody-25810.html
Manaul Link: https://www.elabscience.com/viewpdf-25810-elabscience-E-AB-21221.PDF